研究実績

Google scholar: https://scholar.google.com/citations?user=uTg9qxEAAAAJ&hl=ja

Researchgate: https://www.researchgate.net/profile/Kosuke_Fujishima

Publication

Tretyachenko V, Voráček V, Souček R, Fujishima K, Hlouchová K. CoLiDe: Combinatorial Library Design tool for probing protein sequence space. Bioinformatics, accepted

Jia TZ, 藤島 皓介, 丹羽 達也, 液-液相分離と生命の起源. 生物工学会誌 第98巻 第5号 228–254(2020)

Wang P, Fujishima K, Berhanu S, Kuruma Y, Jia T, Khusnutdinova AN, Yakunin AF, and McGlynn SE. A bi-functional polyphosphate kinase driving NTP regeneration and reconstituted cell-free protein synthesis. ACS Synbio, 9 (1), 36-42. (2019)

Takahagi W, Seo K, Shibuya T, Takano Y, Fujishima K, Saitoh M, Shimamura S, Matsui Y, Tomita M and Takai K. Peptide synthesis under the alkaline hydrothermal conditions on Enceladus. ACS Earth Space Chem, 3, 11, 2559-2568. (2019)

Urbina J, Patil A, Fujishima K, Paulino-Lima IG, Saltikov C, Rothschild LJ. A new approach to biomining: Bioengineering surfaces for metal recovery from aqueous solutions. Sci Rep. 9(1), 16422. (2019)

Fujishima, K., Dziomba, S, Yano H, Kebe SI, Guerrouache M, Carbonnier B, and Rothschild, LJ. The non-destructive separation of diverse astrobiologically relevant organic molecules by customizable capillary zone electrophoresis and monolithic capillary electrochromatography. Int J Astrobiol, 18, 6, 562-574. (2019)

Vecchioni S, Capece MC, Toomey E, Nguyen L, Ray A, Greenberg A, Fujishima K, Urbina J, Paulino-Lima IG, Pinheiro V, Shih J, Wessel G, Wind SJ, Rothschild LJ. Construction and characterization of metal ion-containing DNA nanowires for synthetic biology and nanotechnology. Sci Rep, 9(1), 6942. (2019)

Fujishima, K., Wang, K. M., Palmer, J. A., Abe, N., Nakahigashi, K., Endy, D., and Rothschild, L. J. Reconstruction of cysteine biosynthesis using engineered cysteine-free enzymes. Sci Rep, 8(1), 1776. (2018)

Kaneta A, Fujishima K, Morikazu W, Hori H, Hirata A. The RNA-splicing endonuclease from the euryarchaeaon Methanopyrus kandleri is a heterotetramer with constrained substrate specificity. Nucleic Acids Res. 46(4):1958-1972. (2018)

Froese, T., Campos, J. I., Fujishima, K., Kiga, D., and Virgo, N. Horizontal transfer of code fragments between protocells can explain the origins of the genetic code without vertical descent. Sci Rep, 8(1), 457. (2018)

Kitadai N., Kameya M. and Fujishima K. Origin of the Reductive Tricarboxylic Acid (rTCA) Cycle-Type CO2 Fixation: A Perspective. Life, 7(4), 39. (2017)

Matsubara T., Fujishima K, Saltikov CW., Nakamura S. and Rothschild LJ. Earth analogs for past and future life on Mars: Isolation of perchlorate resistant halophiles from Big Soda Lake. Int J Astrobiology, 1-11. (2016).

Paulino-Lima IG., Fujishima K., Navarrete JU, Galante D, Rodrigues F, Azua-Bustos A. and Rothschild LJ. Extremely high UV-C radiation resistant microorganisms from desert environments with different manganese concentrations. J Photochem Photobiol B, 163:327-36. (2016)

Holm Hansen AC., Paulino-Lima IG., Fujishima K., Rothschild LJ. and Jensen PR. Draft Genome Sequence of Hymenobacter sp. Strain AT01-02, Isolated from a Surface Soil Sample in the Atacama Desert, Chile. Genome Announc, 4(1) e01701-15. (2016)

Fujishima K., Venter C., Wang K., Ferreira R, and Rothschild LJ. An overhang-based exon shuffling method for creating a customized random DNA library. Sci Rep, 5: 9740. (2015)

Ikeda KT., Hirose Y., Hiraoka K, Noro E., Fujishima K., Tomita M. and Kanai A. Identification, expression, and molecular evolution of microRNAs in the "living fossil" Triops cancriformis (tadpole shrimp). RNA, 21(2):230-242. (2015)

Hirata A, Fujishima K, Yamagami R, Kawamura T, Banfield JF, Kanai A. and Hori H. X-ray structure of the fourth type of archaeal tRNA splicing endonuclease: insights into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity Nucleic Acids Res, 40(20):10554-66. (2012)

Sugahara J., Fujishima K., Nunoura T., Takaki Y., Takami H., Takai K., Tomita M. and Kanai A. Genomic Heterogeneity in a Natural Archaeal Population Suggests a Model of tRNA Gene Disruption. PLoS ONE, 7(3):e32504. (2012)

Hamashima K., Fujishima K., Masuda T., Sugahara J., Tomita M. and Kanai A. Nematode-specific tRNAs that decode an alternative genetic code for leucine. Nucleic Acids Res, 40(8):3653-62 (2011)

Murakami S., Fujishima K., Tomita M. and Kanai A. Metatranscriptomic analysis of microbes in an ocean-front deep subsurface hot spring reveals novel small RNAs and type-specific tRNA degradation. Appl Environ Microbiol, 78(4):1015-1022 (2011)

Fujishima K., Sugahara J., Miller CS., Baker BJ., Di Giulio M., Takesue K., Sato A., Tomita M., Banfield JF. and Kanai A. A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity. Nucleic Acids Res, 39(22):9695-704 (2011)

Fujishima K., Sugahara J., Tomita M. and Kanai A. Large-scale tRNA intron transposition in the archaeal order Thermoproteales represents a novel mechanism of intron gain. Mol Biol Evol, 27(10):2233-43 (2010)

Takane K., Fujishima K., Watanabe Y., Sato A., Saito N., Tomita M. and Kanai A. Computational prediction and experimental validation of evolutionarily conserved microRNA target genes in bilaterian animals. BMC Genomics, 11(1):101 (2010).

Kitamura S., Fujishima K., Sato A., Tsuchiya D., Tomita M. and Kanai A. Characterization of RNase HII substrate recognition using RNase HII-Argonaute chimeric enzymes from Pyrococcus furiosus. Biochem J, 426(3):337-44 (2010)

Fujishima K., Sugahara J., Kikuta K., Hirano R., Sato A., Tomita M. and Kanai A. Tri-split tRNA is a transfer RNA made from three transcripts that provides insight into the evolution of fragmented tRNAs in archaea, Proc Natl Acad Sci U S A, 106(8):2683-7 (2009).

Sugahara J., Fujishima K., Morita K., Tomita M. and Kanai A. Disrupted tRNA gene diversity and possible evolutionary scenarios. J Mol Evol, 69(5):497-504 (2009)

Fujishima K., Sugahara J., Tomita M. and Kanai A. Sequence Evidence in the Archaeal Genomes that tRNAs Emerged Through the Combination of Ancestral Genes as 5' and 3' tRNA Halves. PLoS ONE, 25(12):2709-16 (2008)

Sugahara J., Kikuta K., Fujishima K., Yachie N., Tomita M. and Kanai A. Comprehensive analysis of archaeal tRNA genes reveals rapid increase of tRNA introns in the order thermoproteales. Mol Biol Evol, 25(12):2709-16 (2008)

Fujishima K., Komasa M., Kitamura S., Tomita M. and Kanai A. Comparison and characterization of proteomes in the three domains of life using 2D correlation analysis. Progress of Theoretical Physics Supplement, 173:206-18 (2008)

*Fujishima K., Komasa M., Kitamura S., Suzuki H., Tomita M. and Kanai A. Proteome-wide prediction of novel DNA/RNA-binding proteins using amino acid composition and periodicity in the hyperthermophilic archaeon Pyrococcus furiosus. DNA Res, 14(3):91-102 (2007)

Review

Fujishima K and Kanai A. tRNA gene diversity in the three domains of life. Front Genet, (5):142. (2014)

Fujishima K and Kanai A. Split Gene. Brenner’s Encyclopedia of Genetics, 2nd edition, 543-544. (2013)

Book

連載:新分野創造「シンセティック・アストロバイオロジー」 細胞工学,学研メディカル秀潤社

第1回「アストロバイオ×合成生物学」 Vol.34 No.2 (2015)

第2回「火星移住と合成生物学(前編)」Vol.34 No.4 (2015)

第3回「火星移住と合成生物学(後編)」Vol.34 No.7 (2015)

第4回「第二の生命と合成生物学(前編)」Vol.34 No.12 (2015)

第5回「第二の生命と合成生物学(後編)」Vol.35 No.1 (2016)

第6回「生命の起源と合成生物学」Vol.35 No.3 (2016)

おもろいバイオロジー第8回 拝啓,未来のアストロバイオロジストへ 細胞工学 Vol.32 No.12 (2013),学研メディカル秀潤社

Fujishima K, Sugahara J, Kanai A. 三つの遺伝子に断片化したtRNAの発見. 生化学 Vol.82(7):606-12 (2010), 日本生化学会